KBS029: Plant C, N, and P
In use from sometime
see KBS024-prot01.
Abstract
Sampling Frequency: Annually Total carbon and nitrogen contents of plant materials from each plot is measured on vegetation collected for primary productivity sampling with the use of an automated CHN analyzer . After drying at 60 degrees C for 48 h, plant materials are ground to pass through a 0.4 mm mesh using a Wiley Mill and Tecator Impact Pulverizer. Duplicate subsamples (5.0 mg) of the sample are weighed with an electronic microbalance,packed into tin containers, and combusted at 1050 degrees C in an O 2 -enriched atmosphere . Combustion gases are analyzed for C and N with a thermal conductivity detector after separation on a Porapak Q column . Standards compounds includecyclohexanone-2,4-dinitrophenylhydrazone (1990 and prior), acetanilide (1991 to 1997) andphenacetin (1997+) .
Protocol
Author: Tim Bergsma
Edit | Back