Thorn, R. G., C. A. Reddy, D. Harris, and E. A. Paul. 1996. Isolation of saprophytic basidiomycetes from soil. Applied and Environmental Microbiology 62:4288-4292.

Citable PDF link:

A method with the combined advantages of soil particle washing, selective inhibitors, and an indicator substrate was developed to isolate saprophytic basidiomycetes from soil. Organic particles were washed from soil and plated on a medium containing lignin, guaiacol, and benomyl, which reduced mold growth and allowed detection of basidiomycetes producing laccase or peroxidase. The 64 soil samples yielded 67 basidiomycete isolates, representing 51 groups on the basis of morphology and physiology. This method should facilitate investigations into the biodiversity of soil basidiomycetes and yield organisms that are useful in bioremediation of soils contaminated with pesticides or other recalcitrant aromatic compounds.

DOI: 10.1128/AEM.62.11.4288-4292.1996

Associated Treatment Areas:

TDF T1 T2 T3 T4 T5 T6 T7 T8 TCF TSF

Download citation to endnote bibtex

Sign in to download PDF back to index
Sign In