Bogar, G., J. T. Lennon, H. M. Vander Stel, and S. E. Evans. 2025. Microscale assay for the quantification of total polysaccharides to estimate extracellular polymeric substances (EPS) in soil. Journal of Microbiological Methods 239:107324.
Citable PDF link: https://lter.kbs.msu.edu/pub/4267
We present an inexpensive and practical microscale spectrophotometric acid-phenol assay to quantify polysaccharides in aqueous solution. The instant reaction within disposable materials is designed and benchmarked for use in quantifying extracellular polymeric substances (EPS) in soils. Precipitation recovery was high across expected ranges of soil EPS content.
DOI: 10.1016/j.mimet.2025.107324
Associated Treatment Areas:
- Methods
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