Soil Microbial Biomass via Chloroform Fumigation — Main Cropping System Experiment (MCSE)

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Soil microbial biomass carbon and nitrogen as determined by chloroform fumigation – incubation.


This datatable is part of the Microbial Biomass Dynamics dataset.

The purpose of these studies is to determine patterns of microbial biomass dynamics in crop, successional and forest communities.

This project consists of two studies involving microbial biomass determination in soil samples taken from the Main Cropping System Experiment (KBS004): Chloroform Fumigation-Incubation; Soil Microbial Biomass C and N, and Epifluorescence Microscopy and Image Analysis; Soil Microbial Biovolume and C. The data for both studies are generated from subsets of the same soil samples, collected from treatments 1 – 8, CF, DF, and SF. See the descriptor (KBS004) for treatment descriptions. Samples are collected approximately monthly (during ice-free conditions) or annually (varies by year) from 5 sampling stations in each of 6 replicate plots per treatment; exceptions are treatment 8 (four reps), and treatments CF, DF, and SF (three reps each). For experiment descriptions, protocols, and data, see individual datasets.

Samples were collected by K. Klingensmith in 1989 and by S. Halstead in other years. D. Harris performed lab analysis, data entry, data analysis, and data submission. Original data files not available; contact the LTER data manager with questions. The data currently available was derived from web-based files which were last reviewed by D. Harris on 10-01-1997 and may be available from archives.


Experiment: Main Cropping System Experiment (MCSE)
Data available from: April 1989 to May 1996
Dataset: KBS011
Datatable ID: KBS011-001.29
Core Areas Populations,Organic Matter,Disturbance
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Related Tables:
Last Updated 2023-10-24
Variate Description Units
date date of sampling
trt cropping system treatment
rep replicate block
stn replicate station within block
co2 c CO2 mineralized in 10 day incubation of unfumigated soil at 25 Celsius μg/g
co2 f CO2 mineralized in 10 day incubation of chloroform fumigated soil at 25 Celsius μg/g
nc N mineralized in 10 day incubation of unfumigated soil at 25 C μg/g
nf N mineralized in 10 day incubation of chloroform fumigated soil at 25 C μg/g
cfibio c microbial biomass C μg/g
cfibio n microbial biomass N μg/g
Year year in which date occurs

Data Excerpt

date trt rep stn co2_c co2_f nc nf cfibio_c cfibio_n year
1989-04-24 T1 R1 C 146.7 147.1 -2.55 14.32 172.0 37.7 1989.0
1989-04-24 T1 R2 C 193.0 194.0 -1.4 16.26 228.0 44.8 1989.0
1989-04-24 T1 R3 C 163.4 206.0 13.33 12.02 265.0 30.7 1989.0
1989-04-24 T1 R4 C 192.6 181.2 -1.44 15.88 206.0 42.2 1989.0
1989-04-24 T1 R5 C 253.6 244.5 6.09 18.52 281.0 47.7 1989.0
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