LTER: Nitrogen Deposition Study


In use from 2001-01-01 to 2009-12-31


Protocol for Nitrogen application in forest and successional field plots

Fertilized (F) plots are 100 m2 (10m x 10m) each, except in CF3, where the fertilized plot is 6m2(2m x 3m). Annual application rate is 100 kg Nitrogen (N) ha-1 as NH4NO3, equivalent to 1 kg (60 g on CF3) N per plot. The N is added in 3 doses. Each time 16 L (4 L on CF3) of fertilizer solution are added per plot. The 3 doses are applied in the period April thru November (after spring thaw thru litterfall).
Three-fold fertilization (3F) plots are 4m2(2m x 2m) each. The annual application rate is 300 kg N ha-1 added in 4 L per plot solutions.


Preparing the Fertilization solutions

  1. Weigh 8 bags of 980g NH4NO3 (34% N) for 1F, 1 bag of 58.8g NH4NO3 (34% N) for 1F in CF3, and 9 bags of 118g in NH4NO3 (34% N) for 3F.
  2. Mix fertilizer with RO water in 4L flasks with stir bars on stir plate. Add fertilizer slowly to allow it to dissolve.
  3. Pour solutions into 4L or 16L sprayers and carboys for spraying or transport to the field.
  4. Fill the containers for 1F plots to 16L and the containers for CF3-1F and all 3F plots to 4L.

Spraying the plots

Use a 16L backpack sprayer to apply the fertilizer solutions to the 1F plots and a 4L hand sprayer for the 3F plots and 1F in CF3. Divide the 1F plots into quadrants and apply 4 liters of solution to each. In the 3F plots, apply the 4 liters of solution evenly across the plot.


Ammonium nitrate was not available in 8/2003, 10/2006, and 4/2007. Urea (46% N) was used for fertilizing at the same rates of N. The 1F plots had 724.4g Urea applied, except CF3 received 43.5g Urea. The 3F plots received 87.2g Urea/application.

Date modified: Wednesday, Nov 29 2023



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