The Cellulosic Biofuels Diversity Experiment is a long-term study located within the LTER Main Site complex, in which a series of 12 different cropping systems vary in species makeup and nitrogen input. Treatments include continuous corn, corn-soybean, two variations of switchgrass fertilized differently, a C3 – C4 grass-legume mix, and 4 different prairie restorations with 6, 10, 18, or 30 different species native to Michigan. Switchgrass varieties are Cave-in rock (commercial non-native) and Southlow (commercial native). Prairie grasses include switchgrass (Panicum virgatum), big bluestem (Andropogon gerardii), little bluestem (Schizachyrium scoparium), and Indian grass (Sorghastrum nutans).
Treatment plots are 9.1 by 27m (30 x 90 feet) replicated in each of 4 randomized blocks. The study site was established in 2008.