The Great Lakes Bioenergy Research Center (GLBRC) Biofuel Cropping System Experiment (BCSE) was established in 2008 on the KBS GLBRC Intensive field site. Eight different cropping systems are in a randomized complete block design (5 replicate blocs of 30 x 40m plots) that include, in order of plant diversity, continuous corn, a corn-soybean-canola rotation, switchgrass, miscanthus, hybrid poplar, mixed-species native grasses, successional vegetation, and restored prarie. In 2012 the corn-soybean-canola system was removed and replaced by a continuous corn + cover crop system and a corn-soybean + cover crop system with two entry points. Split plots include stover removal (continuous corn only) and nitrogen treatment. A sister experiment in Arlington WI has the same experimental design.