# Equations for Soil Microbial Biovolume and Carbon Calculation

Active

## Abstract

The equations for calculation of soil microbial biovolume and carbon are specified.

## Epifluorescence Microscopy: Equations for Soil Microbial Biovolume and Carbon Calculation

 VARIATE: DEFINITION AND EQUATION: s: Mass of soil per image(µg) where x = 512 * 0.089(µm), y = 768 * 0.089 (µm) for bacterial images; x = 512 * 1.0 (µm), y = 768 * 1.0 (µm) for fungal hyphal images; %H2O = water content of soil sample. bv: Volume of each bacterial cell (µm3 ) d = small diameter of cell l= long diameter of cell The volume (bv) of each bacteria is calculated from measurements of the large, l, and small, d, diameters of each cell by assuming that the cells are prolate spheroids. BAC_NUM: Total bacteria (cells g-1soil) BAC_NUM = count per image / S BAC_VOL: Bacterial biovolume (µm3g-1 soil) BAC_VOL = BAC_C: Bacterial biomass C (µgC g-1soil) BAC_C = BAC_VOL * 0.2 The factor 0.2 used to convert bacterial biovolume to carbon © is thought to be the best available based on a range of estimates available in the literature. hv: Volume of each hyphal fragment (µm3) d = hyphal diameter l = hyphal length Hyphal volume for each fragment is calculated assuming the hyphae are simple cylinders of length l and diameter d. HYP_LEN: Total fungal hyphal length (m g-1 soil) HYP_LEN = *10-6 l = hyphal length HYP_DIAM: Mean fungal hyphal diameter (µm) HYP_VOL: Fungal hyphal biovolume (µm3 g-1 soil) HYP_VOL = HYP_C: Fungal hyphal biomass C (µgC g-1 soil) HYP_C = HYP_VOL * 0.15 The factor 0.15 used to convert fungal biovolumes to carbon © is thought to be the best available based on a range of estimates available in the literature. DMC_C: Total microscopic biomass C (µgC g-1 soil) DMC_C = BAC_C + HYP_C

Date modified: Monday, May 13 2024

Author: